AP BiologyCommon mistakesIons pile up on the side the pump sends them to

Ions pile up on the side the pump sends them to

  1. The idea
  2. Predict
  3. See it
  4. Why
  5. Check

The idea

“The electron transport chain pumps H+ into the matrix, so that's where H+ collects.”

The matrix is where the citric acid cycle makes NADH, so it seems like the place H+ would gather. The chain sends H+ out of it.

The short version

The mistake: The electron transport chain pumps H+ into the matrix, so that's where H+ collects.

What's actually true: The chain pumps H+ out of the matrix into the intermembrane space, which ends up with more H+ and a lower pH.

  1. A pump spends energy to move an ion against its gradient. The ion piles up on the side the pump sends it to.
  2. The chain pumps H+ from the matrix into the intermembrane space. In chloroplasts, a similar chain pumps H+ from the stroma into the thylakoid lumen.
  3. ATP synthase is the way back. H+ flows down its gradient through it, and that flow makes ATP.

On the exam: Ask where the pump sends the ion. That side gets crowded.

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