AP BiologyCommon mistakesIn a chloroplast, H+ pile up inside the thylakoids

In a chloroplast, H+ pile up inside the thylakoids

  1. The idea
  2. Predict
  3. See it
  4. Why
  5. Check

The idea

“Like a mitochondrion, a chloroplast builds its H+ gradient between its two outer membranes.”

You just learned the mitochondrion, with H+ packed between its two membranes. A chloroplast has two envelope membranes too, so the same layout seems obvious.

The short version

The mistake: Like a mitochondrion, a chloroplast builds its H+ gradient between its two outer membranes.

What's actually true: H+ build up in the thylakoid lumen and flow out into the stroma, where ATP is made and the Calvin cycle runs.

  1. Match the parts by job. The thylakoid membrane holds the electron transport chain and ATP synthase, like the inner mitochondrial membrane.
  2. The lumen, the space inside each thylakoid, is like the intermembrane space. In light it fills with H+ and becomes more acidic than the stroma.
  3. The stroma, the fluid around the thylakoids, is like the matrix. ATP synthase releases ATP there, and the Calvin cycle uses it there.

On the exam: H+ pile into the thylakoid lumen. ATP comes out into the stroma. The envelope gap gets none.

Every common AP Biology mistake