AP BiologyCommon mistakesA buffer holds pH where it is

A buffer holds pH where it is

  1. The idea
  2. Predict
  3. See it
  4. Why
  5. Check

The idea

“A buffer pushes the pH up, feeds the reaction, or keeps the temperature steady.”

A buffer comes labeled with a pH, so it's easy to picture it pushing the solution to that number. Its real job starts when acid or base shows up.

The short version

The mistake: A buffer pushes the pH up, feeds the reaction, or keeps the temperature steady.

What's actually true: A buffer resists changes in H+ concentration. It takes up or gives off H+, so the pH stays near where it started.

  1. A buffer is a weak acid mixed with its conjugate base, the same molecule minus one H+.
  2. Added H+ gets bound by the base form. Added OH- takes H+ from the acid form and becomes water. Free H+ barely changes.
  3. In an enzyme lab, a buffer keeps pH a controlled variable as the reaction runs. It doesn't feed the enzyme or touch the temperature.

On the exam: A buffer keeps pH steady by taking up or giving off H+ as acid or base is added.

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